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Please use this identifier to cite or link to this item: http://dspace.univer.kharkov.ua/handle/123456789/7234

Название: Hemoglobin binding to phospholipid membranes as revealed by pyrene fluorescence study
Авторы: Kutsenko, O.K.
Gorbenko, G.P.
Trusova, V.M.
Ключевые слова: hemoglobin
model membranes
protein-lipid complexes
fluorescence spectroscopy
Issue Date: 2011
Издатель: Харьковский Национальный Университет им. В.Н.Каразина
Библиографическое описание: Журнал
Серия/номер: Биофизический вестник;26(1)
Краткий осмотр (реферат): In this work hemoglobin (Hb) association with lipid bilayers was investigated using fluorescent probe pyrene. Model membranes were prepared from zwitterionic lipid phosphatidylcholine (PC), anionic lipid phosphatidylglycerol (PG) and cholesterol (Chol). Hb-lipid binding was followed by the pyrene fluorescence quenching. Hb-induced decrease of pyrene monomer fluorescence was followed by the increase of relative intensities of vibronic bands. Presumably, Hb penetration into the bilayer increases the space between neighbouring lipids and promotes water penetration into the membrane core. Pyrene excimer emission quenching was interpreted in terms of resonance energy transfer. The greatest depth of Hb penetration into the lipid bilayer was observed in PC vesicles. In Chol-containing liposomes sterol condensing effect prevents deep protein penetration into the membrane. PG has an ability to stabilize lipid bilayers due to the ordered state of its lipid tails and H-bonding interactions between lipid molecules. This also can prevent Hb access to the inner membrane regions.
URI: http://dspace.univer.kharkov.ua/handle/123456789/7234
Appears in Collections:Наукові роботи. Радіофізичний факультет

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