Факультет радіофізики, біомедичної електроніки та комп’ютерних систем >
Наукові роботи. Радіофізичний факультет >

Please use this identifier to cite or link to this item: http://dspace.univer.kharkov.ua/handle/123456789/7217

Название: Cytochrome C – cardiolipin interactions: extended lipid anchorage revisited
Авторы: Trusova, V.M.
Ключевые слова: cytochrome c
extended lipid conformation
ionic strength
Issue Date: 2010
Издатель: Харьковский Национальный Университет им. В.Н.Каразина
Библиографическое описание: Журнал
Серия/номер: Биофизический вестник;24(1)
Краткий осмотр (реферат): Resonance energy transfer (RET) from antrylvinyl-labeled (AV) lipids to the heme moiety of cytochrome c (cyt c) has been employed to assess the molecular level details of cyt c interactions with negatively charged lipid membranes composed of phosphatidylcholine (PC) and its mixture with 10 or 20 mol % of cardiolipin (CL). At the lowest ionic strength used here (20 mM) RET profiles from neutral (AV-PC) and anionic (AV-CL) donor were virtually indistinguishable, suggesting that the peculiarities of cyt c association with lipid membranes containing different donors are identical. In contrast, elevating ionic strength up to 40 and 60 mM resulted in expected decrease of energy transfer efficiency in the case of AV-PC containing liposomes, but not for those with AV-CL where RET exhibited an unexpected enhancement with increasing ionic strength. Monte Carlo analysis of the results obtained allowed us to attribute this untypical behavior to the transition of CL into extended lipid conformation. The revealed peculiarities of cyt c – CL interactions are of great interest not only from the viewpoint of regulating cyt c electron transfer and apoptotic propensities but also for elucidating the general mechanisms by which membrane functional activities can be modulated by protein-lipid interactions.
URI: http://dspace.univer.kharkov.ua/handle/123456789/7217
Appears in Collections:Наукові роботи. Радіофізичний факультет

Files in This Item:

File Description SizeFormat
Trusova_63.pdf296,02 kBAdobe PDFThumbnail
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! Яндекс цитирования DSpace Software Copyright © 2002-2010  Duraspace - Feedback